Evaluation of a Rapid High-Performance Liquid Chromatographic Method for Plasma Theophylline Analysis and Its Use in Therapeutic Drug Monitoring

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Abstract
A high-performance liquid chromatographic (HPLC) assay for theophylline [used in the treatment of asthma; a bronchodilator] was developed and evaluated, and interferences were eliminated. The method was compared to an enzyme multiplied immunoassay technique (EMIT) with respect to cost and speed of analysis, recovery of added analyte, precision, accuracy and performace in an external quality assessment scheme. The HPLC method, although less rapid than EMIT, was superior with respect to precision, and was less expensive to perform.