Dissociation-recombination of sunflower seed acid phosphatase
- 30 September 1977
- journal article
- research article
- Published by Springer Nature in Biochemical Genetics
- Vol. 15 (9-10) , 897-901
- https://doi.org/10.1007/bf00483985
Abstract
Formal genetic studies of sunflower (Helianthus annuus) seed acid phosphatase (ACP, E.C. 3.1.3.2) had suggested that the functional enzyme consists of two polypeptide subunits. The dimeric quaternary structure was demonstrated by dissociation-recombination procedures. Dissociation of electrophoretically distinct homodimers was effected upon freezing of extracts in a pH 8–9 buffer containing 1 m NaCl and 0.1 m 2-mercaptoethanol. Reassociation, as indicated by the formation of the hybrid isozyme, occurred during 12 hr dialysis against a pH 7.0 buffer.Keywords
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