Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120
Open Access
- 3 July 2006
- journal article
- research article
- Published by Springer Nature in Retrovirology
- Vol. 3 (1) , 39
- https://doi.org/10.1186/1742-4690-3-39
Abstract
During natural HIV infection, an array of host receptors are thought to influence virus attachment and the kinetics of infection. In this study, to probe the interactions of HIV envelope (Env) with various receptors, we assessed the inhibitory properties of various anti-Env monoclonal antibodies (mAbs) in binding assays. To assist in detecting Env in attachment assays, we generated Fc fusions of full-length wild-type gp120 and several variable loop-deleted gp120s. Through investigation of the inhibition of Env binding to cell lines expressing CD4, CCR5, DC-SIGN, syndecans or combinations thereof, we found that the broadly neutralizing mAb, 2G12, directed to a unique carbohydrate epitope of gp120, inhibited Env-CCR5 binding, partially inhibited Env-DC-SIGN binding, but had no effect on Env-syndecan association. Furthermore, 2G12 inhibited Env attachment to primary monocyte-derived dendritic cells, that expressed CD4 and CCR5 primary HIV receptors, as well as DC-SIGN, and suggested that the dual activities of 2G12 could be valuablein vivofor inhibiting initial virus dissemination and propagation.Keywords
This publication has 77 references indexed in Scilit:
- The C108g Epitope in the V2 Domain of gp120 Functions as a Potent Neutralization Target When Introduced into Envelope Proteins Derived from Human Immunodeficiency Virus Type 1 Primary IsolatesJournal of Virology, 2005
- Cryptic Nature of a Conserved, CD4-Inducible V3 Loop Neutralization Epitope in the Native Envelope Glycoprotein Oligomer of CCR5-Restricted, but Not CXCR4-Using, Primary Human Immunodeficiency Virus Type 1 StrainsJournal of Virology, 2005
- Binding of the 2F5 Monoclonal Antibody to Native and Fusion-Intermediate Forms of Human Immunodeficiency Virus Type 1 gp41: Implications for Fusion-Inducing Conformational ChangesJournal of Virology, 2004
- Antibody neutralization and escape by HIV-1Nature, 2003
- Envelope-Dependent, Cyclophilin-Independent Effects of Glycosaminoglycans on Human Immunodeficiency Virus Type 1 Attachment and InfectionJournal of Virology, 2002
- cisExpression of DC-SIGN Allows for More Efficient Entry of Human and Simian Immunodeficiency Viruses via CD4 and a CoreceptorJournal of Virology, 2001
- Postattachment Neutralization of a Primary Strain of HIV Type 1 in Peripheral Blood Mononuclear Cells Is Mediated by CD4-Specific Antibodies But Not by a Glycoprotein 120-Specific Antibody that Gives Potent Standard NeutralizationAIDS Research and Human Retroviruses, 2001
- Binding of Recombinant Feline Immunodeficiency Virus Surface Glycoprotein to Feline Cells: Role of CXCR4, Cell-Surface Heparans, and an Unidentified Non-CXCR4 ReceptorJournal of Virology, 2001
- Increased Infectivity of HIV Type 1 Particles Bound to Cell Surface and Solid-Phase ICAM-1 and VCAM-1 through Acquired Adhesion Molecules LFA-1 and VLA-4AIDS Research and Human Retroviruses, 2000
- CD4-dependent, antibody-sensitive interactions between HIV-1 and its co-receptor CCR-5Nature, 1996