Improved detection of hepatitis B surface antigen (HBsAg) in blood donors by monoclonal radioimmunoassay
- 2 January 1985
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 25 (1) , 10-14
- https://doi.org/10.1046/j.1537-2995.1985.25185116491.x
Abstract
The performance of a monoclonal radioimmunoassay (M-RIA) with enhanced sensitivity and high specificity for hepatitis B surface antigen (HBsAg) was evaluated in blood donors. The results were compared to a conventional RIA that used polyvalent antibodies (P-RIA). Analysis of 6409 American blood donors not reactive by P-RIA revealed an additional 1.4 HBsAg-positive donors per 1000 by M-RIA, or an approximate 60%, improvement in HBsAg detection rate. In 995 Israeli blood donors negative by P-RIA, 11 additional HbsAG-positive donors were identified. The 55% improvement in detection rate was similar to that observed with American blood donors. Since several of the newly identified HBsAG-positive blood donors had antibodies to the core antigen (anti-HBc) as the only serologic evidence of recent or past hepatitis B exposure, an additional 68 anti-HBc-positive individuals were studied with the M-RIA. Twenty-six percent (18/68) reacted only by M-RIA and not by P-RIA. Apparently, there are blood donors with HbsAG undetectable by P-RIA.This publication has 14 references indexed in Scilit:
- Mouse Monoclonal Anti-HBs and Its Use in the Screening of Donated Blood by ElisaVox Sanguinis, 1984
- Monoclonal Antibodies as Diagnostic Probes in the Etiology of HepatitisSeminars in Liver Disease, 1984
- Monoclonal Antibodies to Hepatitis B Surface Antigen (HBsAg) as a Tool for the Automated HBsAg ScreeningVox Sanguinis, 1983
- Is the Use of the Term ‘Posttransfusion Hepatitis Type B’ in Its Conventional Sense Still Justifiable?1Vox Sanguinis, 1983
- Monoclonal radioimmunoassays for hepatitis B surface antigen: demonstration of hepatitis B virus DNA or related sequences in serum and viral epitopes in immune complexes.Proceedings of the National Academy of Sciences, 1982
- A rapid one-step radiometric assay for hepatitis B surface antigen utilising monoclonal antibodiesJournal of Immunological Methods, 1982
- DEMONSTRATION OF PREVIOUSLY UNDETECTED HEPATITIS B VIRAL DETERMINANTS IN AN AUSTRALIAN ABORIGINAL POPULATION BY MONOCLONAL ANTI-HBs ANTIBODY RADIOIMMUNOASSAYSThe Lancet, 1982
- Monoclonal IgM radioimmunoassay for hepatitis B surface antigen: high binding activity in serum that is unreactive with conventional antibodies.Proceedings of the National Academy of Sciences, 1982
- Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies.Proceedings of the National Academy of Sciences, 1981
- Anticore Antibody Screening of Transfused BloodVox Sanguinis, 1978