MUCOSAL GLYCOPROTEINS IN CARCINOMA CELLS OF GASTROINTESTINAL TRACT, AS DETECTED BY IMMUNOFLUORESCENCE TECHNIQUE

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Abstract
Antisera, obtained by Immunization of human or pocine gastric muco‐sa‐specific and intestinal mucosa‐specific glycoprotein into rabbits, were employed for the immunofluorescence staining of carcinoma cells in the gastrointestinal tract. The undifferentiated carcinoma cells, diffusely and dissociatedly proliferating in the mucosa as well as in the submucosal and muscular layer, fluoresced intensely for both antisera. On the other hand, the well‐differentiated ones of adenocarcinoma type were devoid of fluorescence. Fluorescence appeared where the ordered glandular pattern became disarranged. Carcinoma cells metastasizing into serous effusion singly or in small clusters, fluoresced well. The usefullness of this immunofluorescence technique in the cytologic diagnosis of carcinoma cells in effusions was ascertained.