Stereochemical course and structure of the products of the enzymic action of endo-1,3-1,4-β-d-glucan 4-glucanohydrolase from Bacillus licheniformis
- 15 December 1993
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 296 (3) , 753-758
- https://doi.org/10.1042/bj2960753
Abstract
The stereochemical course of the reaction catalysed by endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase (EC 3.2.1.73) has been determined by 1H n.m.r. The enzyme-catalysed hydrolysis of barley beta-glucan proceeds with overall retention of the anomeric configuration, indicating that the enzyme operates through a double-displacement mechanism. The structures of the final oligosaccharide products, 3-beta-O-cellobiosyl D-glucopyranoside and 3-beta-O-cellotriosyl D-glucopyranoside, have been completely assigned by 1H- and 13C-n.m.r. spectroscopy.Keywords
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