Direct immunoperoxidase staining for regan isoenzyme of alkaline phosphatase in human tumor tissues
- 1 September 1976
- Vol. 38 (3) , 1237-1246
- https://doi.org/10.1002/1097-0142(197609)38:3<1237::aid-cncr2820380325>3.0.co;2-2
Abstract
Immunoperoxidase staining for Regan isoenzyme of alkaline phosphatase was performed on cryostat sections of five human tumor tissues. With a direct immunoperoxidase staining for the localization of Regan isoenzyme at the light and electron microscope levels, sections previously fixed with 0.05 M phosphate-buffered 4% paraformaldehyde were reacted with rabbit antisera to human placenta alkaline phosphatase conjugated to horseradish peroxidase. Comparison of conventional histochemistry and immunohistochemistry for Regan isoenzyme indicated that strong specific immunoperoxidase staining appeared on the cell membrane surface, and a diffuse one, in the cytoplasm of lung and colon cancer tissue cells showing L-phenylalanine-sensitive alkaline phosphatase. No immunoperoxidase reaction was obtained in tumor cells showing sensitivity to L-homoarginine or lacking alkaline phosphatase activity.This publication has 24 references indexed in Scilit:
- Demonstration of lysosomal and extralysosomal sites for acid phosphatase in mouse kidney tubule cells with p-nitrophenylphosphate lead-salt technique.Journal of Histochemistry & Cytochemistry, 1975
- PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATIONJournal of Histochemistry & Cytochemistry, 1974
- PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPYJournal of Histochemistry & Cytochemistry, 1974
- CHARACTERIZATION OF TWO HELA SUBLINES: TCRC-1 PRODUCES REGAN ISOENZYME AND TCRC-2, NON-REGAN ISOENZYMEThe Journal of cell biology, 1974
- ELECTRON MICROSCOPIC STUDY OF THE ADRENOCORTICOTROPIN-PRODUCING CELL WITH THE USE OF UNLABELED ANTIBODY AND THE SOLUBLE PEROXIDASE-ANTIPEROXIDASE COMPLEXJournal of Histochemistry & Cytochemistry, 1972
- l-HOMOARGININE, A SPECIFIC INHIBITOR OF LIVER-TYPE ALKALINE PHOSPHATASE, APPLIED TO THE RECOGNITION OF LIVER-TYPE ENZYME ACTIVITY IN RAT INTESTINEJournal of Histochemistry & Cytochemistry, 1972
- L-leucine sensitive, heat-stable alkaline-phosphatase isoenzyme detected in a patient with pleuritis carcinomatosaClinica Chimica Acta; International Journal of Clinical Chemistry, 1970
- ULTRASTRUCTURAL LOCALIZATION OF INTRACELLULAR IMMUNE GLOBULINS IN PLASMA CELLS AND LYMPHOBLASTS BY ENZYME-LABELED ANTIBODIESJournal of Histochemistry & Cytochemistry, 1969
- Electron microscopical observations on the surface coating of human blood plateletsJournal of Ultrastructure Research, 1968
- THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUEJournal of Histochemistry & Cytochemistry, 1966