The molecular structure of a rapidly formed oligomeric adenosine tetraphosphate derivative from rat heart
- 15 March 1986
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 234 (3) , 623-627
- https://doi.org/10.1042/bj2340623
Abstract
The inability to account for large systematic variations with time in soluble adenine nucleotides in perfused rat hearts [Bates, Perrett & Mowbray (1978) Biochem. J. 176, 485-493; Mowbray, Bates & Perrett (1981) FEBS Lett. 131, 55-59; Mowbray, Perrett & Bates (1984) Int. J. Biochem. 16, 889-894] led us to show that the soluble nucleotides are in rapid equilibrium with some hitherto unrecognized trichloroacetic acid/methanol-precipitable highly phosphorylated heteropolymeric form [Mowbray, Hutchinson, Tibbs & Morris (1984) Biochem. J. 223, 627-632]. Selective digestion coupled to chromatographic analysis together with m.s. and 31P-n.m.r. spectrometry have now been used to show that the likely structure for a purified oligomer that is in specific-radioactivity equilibrium with tissue ATP is 3-phospho-[glyceroyl-gamma-triphosphoroyl-5′-adenosine-3′-3- phospho]4 glyceroyl-gamma-triphosphoroyl-5′-adenosine.This publication has 13 references indexed in Scilit:
- A possible adenine nucleotide storage form in normal and ischaemic rat heartBioscience Reports, 1985
- The discovery of a rapidly metabolized polymeric tetraphosphate derivative of adenosine in perfused rat heartBiochemical Journal, 1984
- In perfused rat hearts ischaemia promotes the reversible conversion of appreciable quantities of soluble adenine nucleotides to a stable trichloroacetic acid-precipitable formBioscience Reports, 1981
- Inversely related oscillations in the contents of cyclic GMP and the total adenine nucleotides in steady‐state perfused rat heartsFEBS Letters, 1981
- Systematic variations in the content of the purine nucleotides in the steady-state perfused rat heart. Evidence for the existence of controlled storage and release of adenine nucleotidesBiochemical Journal, 1978
- pppA2'p5'A2'p5'A: an inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells.Proceedings of the National Academy of Sciences, 1978
- Evidence that the coupling of histone gene expression and DNA synthesis in HeLa S3 cells is not mediated at the transcriptional levelBiochemical and Biophysical Research Communications, 1977
- Quantitative thin-layer chromatography of ATP and the products of its degradation in meat tissueJournal of Chromatography A, 1974
- Detection of Sugars on Paper ChromatogramsNature, 1950
- Separation of the Phosphoric Esters on the Filter Paper ChromatogramNature, 1949