An Endo-(l → 6)-β-d-glucanase ofFlavobacteriumM64 Hydrolyzing the Octasaccharide Repeating Unit of Succinoglycan to Two Tetrasaccharides

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Abstract
An endo-(l → 6)-β-d-glucanase capable of hydrolyzing octasaccharide to two tetrasaccharides was isolated from cells of Flavobacterium M64. The octasaccharide represents the repeating unit of succinoglycan (SG-D). One tetrasaccharide was composed of d-glucose, succinic acid and pyruvic acid (4:1:1, molar ratio), and the other was composed of d-glucose and d-galactose (3:1, molar ratio). This enzyme hydrolyzed the (l → 6)-β-d-glucosidic linkage adjacent to the (1 → 6)-linked β-d-glucose residue in the octasaccharide repeating unit of succinoglycan and also hydrolyzed the octasaccharide repeating units of similar polysaccharides produced by many strains of Agrobacterium and Rhizobium species.