Localization of a regulatory region on the 5′‐untranslated region of human hepatoma HepG2 γ‐glutamyltransferase mRNA and response to dexamethasone and antisense oligonucleotide treatment

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Abstract
We are reporting the functional analysis of the 5′-untranslated region (5′UTR) of human hepatoma HepG2 γ-glutamyltransferase (GGT) mRNA. Transient expression of hybrid GGT-luciferase reporter gene mutants in HepG2 shows that this 5′UTR acts as a tissue-specific translational enhancer. A domain of 173 bases containing a steroid hormone response element (HRE) is responsible for the enhancing effect, which can be amplified by addition of dexamethasone at 10−6 M. Furthermore, the regulatory role of the 5′UTR is demonstrated by interaction with sense and antisense oligonucleotides.