Production of "rapid-harvest" Moloney murine leukemia virus by continuous cell culture on synthetic capillaries

Abstract
Moloney murine leukemia virus was harvested automatically within 60 min of release from chronically infected NIH/3T3 cells (clone 1) cultured on bundles of synthetic capillaries. Production of virus as measured by a determination of reverse transcriptase activity and by the XC syncytia assay demonstrated that highly infectious "rapid-harvest" virus was recovered from NIH/3T3 cells (clone 1) grown for periods of up to 10 days.