Regulation of surface CD163 expression and cellular effects of receptor mediated hemoglobin‐haptoglobin uptake on human monocytes and macrophages

Abstract
Local dysregulation of iron metabolism is suggested to contribute to atherosclerotic lesion development through hemoglobin scavenging pathways. We evaluated the effects of CD163‐mediated uptake of hemoglobin‐haptoglobin (HbHp) complexes on surface CD163 and intracellular heme oxygenase‐1 expression and the secretion of pro‐ and antiinflammatory cytokines by macrophages. We found that increased availability of HbHp complexes triggers the upregulation of surface CD163, and also results in a dose‐dependent secretion of IL‐6 and IL‐10. © 2006 International Society for Analytical Cytology