THE MECHANISM OF ALLANTOIN DEGRADATION BY A PSEUDOMONAS
- 1 November 1954
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 68 (5) , 598-603
- https://doi.org/10.1128/jb.68.5.598-603.1954
Abstract
Cell suspensions and cell free extracts of Pseudomonas, strain 2RCC-1, isolated by enrichment culture techniques, carry out the aerobic degradation of allantoin to urea, carbon dioxide, and water in the following manner allantoin[forward arrow] allantoic acid[forward arrow] glyoxylic acid + urea; glyoxylic acid[forward arrow] formate + CO2; formate [forward arrow] CO2 + H2O. Thiamin pyrophosphate and Mg++ or Mn++ are required for oxidation of glyoxylate to formate and CO2. Tracer experiments demonstrated that the alpha carbon of glyoxylate is converted to CO2, and the carboxyl carbon to formate.Keywords
This publication has 18 references indexed in Scilit:
- The mechanism of allantoin catabolism by yeastArchives of Biochemistry and Biophysics, 1953
- Studies of glycine oxidation in rat tissuesArchives of Biochemistry and Biophysics, 1953
- THE UTILIZATION OF 2-C14-l-THREONINE FOR THE SYNTHESIS OF URIC ACIDJournal of Biological Chemistry, 1952
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- Glycerine Determination in TissuesAnalytical Chemistry, 1951
- COENZYME A FUNCTION IN AND ACETYL TRANSFER BY THE PHOSPHOTRANSACETYLASE SYSTEMJournal of Biological Chemistry, 1951
- COENZYME A DEPENDENCE AND ACETYL DONOR FUNCTION OF THE PYRUVATE-FORMATE EXCHANGE SYSTEMJournal of Biological Chemistry, 1950
- THE SEPARATION AND IDENTIFICATION OF KETOACIDS BY FILTER PAPER CHROMATOGRAPHYJournal of the American Chemical Society, 1950
- Preparation of Cell-free Bacterial Extracts with Powdered AluminaJournal of General Microbiology, 1948
- Microdetermination of Glycolic and Oxalic AcidsIndustrial & Engineering Chemistry Analytical Edition, 1943