Synthesis of rabbit β-globin in cultured monkey kidney cells following infection with a SV40 β-globin recombinant genome
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 277 (5692) , 108-114
- https://doi.org/10.1038/277108a0
Abstract
Rabbit .beta.-globin complementary (c)DNA was inserted into SV-40 DNA in place of the gene coding for the virus'' major capsid protein, VP1. The recombinant genome, SVGT5-Ra.beta.G, multiplies efficiently in [African green monkey kidney] CV1 monkey kidney cell cultures and is transcribed to yield cytoplasmic, polyadenylated hybrid mRNA containing the .beta.-globin coding sequence. Cells propagating SVGT5-Ra.beta.G produce substantial quantities of rabbit .beta.-globin polypeptide.Keywords
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