Probing In Vivo Promoter Activities in Mycoplasma pneumoniae : a System for Generation of Single-Copy Reporter Constructs
Open Access
- 1 February 2006
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 72 (2) , 1696-1699
- https://doi.org/10.1128/aem.72.2.1696-1699.2006
Abstract
The nucleotide sequences that control transcription initiation and regulation in Mycoplasma pneumoniae are poorly understood. In this work, we developed a lacZ -based reporter plasmid that can be used to integrate fusions of promoter fragments to a promoterless lacZ gene into the chromosome of M. pneumoniae .Keywords
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