Duration of invasiveness ofEntamoeba histolyticamaintainedin vitro

Abstract
The infectivity and invasiveness of a single strain ofEntamoeba histolyticato young rats has been studied over a period of 12 years. The virulence was slowly lost over a period of months or years of cultivationin vitro.After four to six liver passages, virulence was again established and again declined in culture. This was repeated on three occasions. Each time the strain lost virulence, the capacity to invade the mucosa was lost before the amoebae became non-infective to young rats. The duration of experimentally acquired virulence was progressively reduced after each succeeding series of liver passages.

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