CLOSE RELATIONSHIP OF THE MAJOR EXCRETED PROTEIN OF TRANSFORMED MURINE FIBROBLASTS TO THIOL-DEPENDENT CATHEPSINS
- 1 September 1986
- journal article
- research article
- Vol. 46 (9) , 4590-4593
Abstract
Complementary DNA clones corresponding in 638 nucleotides of the messenger RNA encoding the major portion of murine major excreted protein have been isolated and sequenced. The amino acid sequence of a part of the murine major excreted protein deduced from the DNA sequence reveals substantial and significant homology with the cysteine proteases actinidin, rat cathepsin H, and papain. Since the amount of murine major excreted protein secreted by cultured cells is often enhanced by transformation, it is implicated in oncogenic phenomena and may play a role in the metastatic process by virtue of its proteolytic activity.This publication has 17 references indexed in Scilit:
- Thiol proteasesJournal of Molecular Biology, 1985
- Malignant transformation and tumor promoter treatment increase levels of a transcript for a secreted glycoprotein.Molecular and Cellular Biology, 1985
- Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.Proceedings of the National Academy of Sciences, 1983
- Homology of amino acid sequences of rat liver cathepsins B and H with that of papain.Proceedings of the National Academy of Sciences, 1983
- Proteolytic activation of calcium-activated, phospholipid-dependent protein kinase by calcium-dependent neutral protease.Journal of Biological Chemistry, 1983
- Identification of a BALB/c-3T3 cell protein modulated by platelet-derived growth factor.Molecular and Cellular Biology, 1983
- The predominant secreted protein of transformed murine fibroblasts carries the lysosomal mannose 6-phosphate recognition marker.Journal of Biological Chemistry, 1982
- Stimulation of the release of two glycoproteins from mouse 3T3 cells by growth factors and by agents that increase intralysosomal pHBiochemical and Biophysical Research Communications, 1981
- Tumor promoters induce the synthesis of a secreted glycoprotein in mouse skin and cultured primary mouse epidermal cellsCarcinogenesis: Integrative Cancer Research, 1981
- Transformation-dependent secretion of a low molecular weight protein by murine fibroblasts.Proceedings of the National Academy of Sciences, 1978