Efficient plant regeneration from Indica (group 1) rice protoplasts of one advanced breeding line and three varieties
- 1 January 1995
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 15 (1-2) , 68-71
- https://doi.org/10.1007/bf01690256
Abstract
Regenerable embryogenic suspensions were established from one Indica (group 1) rice advanced breeding line and 9 Indica (group 1) rice varieties in 6–8 weeks. Four were chosen for protoplast culture and plant regeneration. About 4–7×107 protoplasts were isolated from one gram of 8-week-old cell suspension. High plating efficiency (30.5%) and colony formation (13.7%) were obtained using nurse culture methods. A high plant regeneration frequency (67.5%) was observed for line IR57311-95-2-3. In total, 322 plants were regenerated. All the regenerated plants were fertile.Keywords
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