Fertile Indica rice plants regenerated from protoplasts isolated from microspore derived cell suspensions
- 1 September 1990
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 9 (5) , 253-256
- https://doi.org/10.1007/bf00232295
Abstract
Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is described. Regenerating protoplasts could readily be isolated from 5–12 months old cell suspensions showing visible colony formation in the range of 180–1050 colonies/106 protoplasts after about one month in culture. More than 100 independent green plantlets were regenerated via secondary embryogenesis from ca 20×106 protoplasts. Out of 32 plants grown to maturity under greenhouse conditions 24 were fertile.This publication has 9 references indexed in Scilit:
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