MUTATIONAL HETEROZYGOTES IN BACTERIOPHAGES
- 1 October 1959
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 45 (10) , 1507-1515
- https://doi.org/10.1073/pnas.45.10.1507
Abstract
When bacteria infected with T2 or T4 bacteriophages are exposed to the mutagen 5-bromodeoxyuridine, some of the resultant mutant phages are heterozygous, giving rise to mixed clones of mutant and nonmutant progeny. The proportion of heterozygotes among mutants rises to at least 80% when progeny phages are harvested very shortly after addition of the mutagen. This proportion is reduced by prolonged exposure of the infected bacteria to 5-bromodeoxyuridine. These findings are interpreted to mean that the mutational heterozygosity of a phage particle resides in a double stranded deoxyribonucleic acid (DNA) molecule which contains one mutant and one nonmutant polynucleotide chain.Keywords
This publication has 13 references indexed in Scilit:
- THE DIFFERENCE BETWEEN SPONTANEOUS AND BASE-ANALOGUE INDUCED MUTATIONS OF PHAGE T4Proceedings of the National Academy of Sciences, 1959
- INDUCTION OF SPECIFIC MUTATIONS WITH 5-BROMOURACILProceedings of the National Academy of Sciences, 1958
- Studies in Mutagenesis: Production of Bacteriophage Mutants by a Disturbance of Deoxyribonucleic Acid MetabolismNature, 1956
- [Early lysis produced by chloroform in bacteria infected by bacteriophage].1956
- FINE STRUCTURE OF A GENETIC REGION IN BACTERIOPHAGEProceedings of the National Academy of Sciences, 1955
- [Not Available].1954
- Incorporation of halogenated pyrimidines into the deoxyribonucleic acids of Bacterium coli and its bacteriophages.1954
- NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH BACTERIOPHAGE T2The Journal of general physiology, 1953
- THE STRUCTURE OF DNACold Spring Harbor Symposia on Quantitative Biology, 1953
- GENETIC RECOMBINATION AND HETEROZYGOSIS IN BACTERIOPHAGECold Spring Harbor Symposia on Quantitative Biology, 1951