FRACTIONATED POPULATIONS OF NORMAL HUMAN MARROW-CELLS RESPOND TO BOTH HUMAN COLONY-STIMULATING FACTORS WITH GRANULOCYTE-MACROPHAGE ACTIVITY
- 1 January 1985
- journal article
- research article
- Vol. 13 (9) , 956-962
Abstract
Populations of normal human colony-forming cells (blast cells) and cluster-forming cells (promyelocytes-myelocytes) were obtained from bone marrow by using the monoclonal antibody WEM G11 and the fluorescence-activated cell sorter (FACS). Both populations were shown to be responsive to both human colony-stimulating factors (CSFs) with granulocyte-macrophage activity (CSF.alpha. and CSF.beta.), with the cluster-forming cell population being more responsive to CSF.beta. than the colony-forming cell population. The clonal proliferation of promyelocytes-myelocytes was transient, and the clones generated were of subcolony size (less than 40 cells) regardless of the CSF used. Clone transfer experiments demonstrated that progeny of promyelocytes-myelocytes initiated using one stimulus (CSF.alpha. or CSF.beta.) were also responsive to the other stimulus.This publication has 24 references indexed in Scilit:
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