THE EFFECT OF POSTFUSION CELL-DENSITY ON ESTABLISHMENT OF HYBRIDOMAS
- 1 January 1988
- journal article
- research article
- Vol. 34 (1) , 18-22
Abstract
The effect of postfusion cell density on establishment of hybridomas in primary microculture was studied. Following fusion of BALB/c spleen and NSO myeloma cells in the ratio of 5:1 we demonstrated that seeding the cells at a concentration of about 20-25 .times. 104 spleen cells per well resulted in maximal number of wells containing only one growing hybridoma clone (40.1-43.7% plated wells). Importance of generation of microculture with one clone for the effectiveness of preparation of mAb-secreting hybridomas is discussed.This publication has 4 references indexed in Scilit:
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- Improved fusion technique. II. Stability and purity of hybrid clonesJournal of Immunological Methods, 1984
- PRODUCTION OF MONOCLONAL-ANTIBODIES WITH HEMAGGLUTINATION-INHIBITION ACTIVITY TO THE SKALICA STRAIN FROM THE TICK-BORNE ENCEPHALITIS COMPLEX1983
- Improved fusion technique. I. Human umbilical cord serum, a new and potent growth promoter, compared with other B cell and hybridoma activatorsJournal of Immunological Methods, 1983