YEAST MESSENGER-RNA SPLICING INVITRO
- 1 January 1985
- journal article
- research article
- Vol. 260 (27) , 4780-4792
Abstract
Synthetic actin and CYH2 pre-mRNAs containing a single intron are accurately spliced in a soluble whole cell extract of yeast. Splicing in vitro requires ATP. The excised intron is released as a lariat in which an RNA branch connects the 5'' end of the molecule to the last A in the "intron conserved sequence" UACUAAC. Two other discrete RNA species produced during splicing in vitro may represent reaction intermediates: free, linear exon 1 and a form of the intron lariat extending beyond the 3'' splice site to include exon 2. Both lariat forms correspond to molecules previously shown to be produced during yeast pre-mRNA splicing in vivo.This publication has 33 references indexed in Scilit:
- Yeast is unable to excise foreign intervening sequences from hybrid gene transcripts.Proceedings of the National Academy of Sciences, 1983
- Polyadenylylated nuclear RNA contains branchesProceedings of the National Academy of Sciences, 1983
- Mechanism of action of a yeast RNA ligase in tRNA splicingPublished by Elsevier ,1983
- Transformation of intact yeast cells treated with alkali cationsJournal of Bacteriology, 1983
- Structural characterization and in vitro processing of Escherichia coli ribosomal RNA transcripts containing 5′ triphosphates, leader sequences, 16 S rRNA, and spacer tRNAsJournal of Molecular Biology, 1980
- Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences, 1980
- Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences, 1980
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Micro thin-layer techniques for rapid sequence analysis of 32P-labeled RNA: Double digestion and pancreatic ribonuclease analysesAnalytical Biochemistry, 1977
- Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedureAnalytical Biochemistry, 1976