Antibodies to Sperm-Associated Antigens Detected by Solid Phase Assays1
- 1 February 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 26 (1) , 140-146
- https://doi.org/10.1095/biolreprod26.1.140
Abstract
Solid phase assays using radiolabeled (RIA) or enzyme-linked (ELISA) antibodies are described for the quantitation of antibodies to sperm-associated antigens. Antibodies were detected in serum from rabbits immunized with human sperm emulsified in Freund''s complete adjuvant and in the plasmas or sera of vasectomized males and patients with suspected antibody-mediated infertility. The RIA and ELISA assays are comparable to each other in sensitivity and specificity, rapid in execution and inexpensive.This publication has 9 references indexed in Scilit:
- Immunologic Infertility: Identification of Patients with Antisperm AntibodyNew England Journal of Medicine, 1980
- DEMONSTRATION OF IGG FC-RECEPTORS ON SPERMATOZOA AND THEIR UTILIZATION FOR THE DETECTION OF CIRCULATING IMMUNE-COMPLEXES IN HUMAN-SERUM1980
- Iodination of Rabbit Sperm Plasma Membrane: Relationship of Specific Surface Proteins to Epididymal Function and Sperm Capacitation1Biology of Reproduction, 1979
- Methods for binding cells to plastic: Application to a solid-phase radioimmunoassay for cell-surface antigensJournal of Immunological Methods, 1979
- ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION OF ANTI-SPERM ANTIBODIES1979
- Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3α,6α-diphenylglycolurilBiochemistry, 1978
- Antigenic Status of Semen from the Viewpoints of the Female and MaleFertility and Sterility, 1978
- A Method of Trace Iodination of Proteins for Immunologic StudiesInternational Archives of Allergy and Immunology, 1966
- The hydrolysis of rabbit γ-globulin and antibodies with crystalline papainBiochemical Journal, 1959