Initial use of a broth microdilution method suitable for in vitro testing of fungal isolates in a clinical microbiology laboratory
- 1 July 1995
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 33 (7) , 1884-9
- https://doi.org/10.1128/jcm.33.7.1884-1889.1995
Abstract
Antifungal susceptibility testing methods currently lack a standardized procedure. Many factors, such as inoculum preparation, inoculum density, medium selection, pH, incubation time and temperature, and endpoint determination, affect results. We developed a workable procedure for fungal susceptibility testing, with a microtiter method based upon modifications of the proposed guidelines from the National Committee for Clinical Laboratory Standards, using two different growth media. For this procedure, the microtiter tray is prepared as a panel of 6 drugs (amphotericin B, flucytosine, fluconazole, ketoconazole, miconazole, and itraconazole) alone and in combination with amphotericin B. Eagle's minimal essential medium and RPMI 1640 are the two growth media. Two separate susceptibility trays are inoculated for each sensitivity test, with one tray incubated at 30 degrees C and the other incubated at 35 degrees C. After 48 h of growth, results for both temperatures and both media are recorded and interpreted. The four test environments (two media each at two temperatures) provided growth for 100 of the first 104 organisms that were submitted for testing. This approach provides a workable methodology for routine antifungal susceptibility testing in a clinical microbiology laboratory setting.Keywords
This publication has 14 references indexed in Scilit:
- Resistance of Candida species to fluconazoleAntimicrobial Agents and Chemotherapy, 1995
- Azole-Resistant Candida albicans: Report of Two Cases of Resistance to Fluconazole and ReviewClinical Infectious Diseases, 1994
- In vitro activity of itraconazole against fluconazole-susceptible and -resistant Candida albicans isolates from oral cavities of patients infected with human immunodeficiency virusAntimicrobial Agents and Chemotherapy, 1994
- Susceptibility testing of fungi: current status of the standardization processAntimicrobial Agents and Chemotherapy, 1993
- Antifungal susceptibility testingClinical Microbiology Reviews, 1993
- Susceptibility testing of Candida species for Auconazole: the role of buffering in the agar dilution assayMycoses, 1993
- Evolving Role of Flucytosine in Immunocompromised Patients: New Insights into Safety, Pharmacokinetics, and Antifungal TherapyClinical Infectious Diseases, 1992
- Amphotericin B: current understanding of mechanisms of actionAntimicrobial Agents and Chemotherapy, 1990
- In vitro activities of amphotericin B in combination with four antifungal agents and rifampin against Aspergillus sppAntimicrobial Agents and Chemotherapy, 1984
- A Pharmacologic Guide to the Clinical Use of Amphotericin BThe Journal of Infectious Diseases, 1969