Evidence for Two Adhesive Antigens on the K99 Reference Strain Escherichia coli B41

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Abstract
Immunoelectrophoresis and hemagglutination studies with a K99 antigen extract from the reference strain E. coli B41 (01O1:K99) demonstrated both anionic and cationic mannose-resistant hemagglutinins for horse red blood cells. Neither hemagglutinin was produced by bacteria grown at 18.degree. C. Antibodies to the cationic hemagglutinin were demonstrated in antisera to all the K99-positive E. coli strains in every serogroup examined, but antibodies to the anionic hemagglutinin were only detected in antisera to E. coli strains from the 09 and 01O1 serogroups. Inhibition of E. coli B41 adhesion to calf brush borders and indirect immunofluorescent staining indicated that the anionic hemagglutinin also exhibited adhesive properties.

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