Ultraviolet Irradiation Transforms C3H10T1/2 Cells to a Unique, Suppressible Phenotype
- 12 December 1986
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 234 (4782) , 1385-1388
- https://doi.org/10.1126/science.3787250
Abstract
Transformation of C3H10T1/2 cells by exposure to ultraviolet (UV) irradiation followed by tetradecanoyl phorbol acetate (TPA) has been used as a model of two-stage carcinogenesis. However, cells cloned from UV-TPA-induced foci (UV-TDTx cells) had a unique phenotype. Cloned UV-TDTx cells appeared transformed in pure culture but were unable to form focic when cocultured with C3H10T1/2 cells. However, in the presence of TPA, UV-TDTx cells from foci in mixed culture with C3H10T1/2 cells. This phenotype was the only one observed for UV-TPA transformants. These data suggest that (i) communal suppression of cell division is a discrete phenomenon that must be overcome as one step in the multistage process of transformation, and (ii) this protocol permits the routine isolation of transformed cells responsive to density-dependent growth suppression.This publication has 11 references indexed in Scilit:
- Growth inhibition of transformed cells correlates with their junctional communication with normal cellsPublished by Elsevier ,1986
- Elimination of Metabolic Cooperation in Chinese Hamster Cells by a Tumor PromoterScience, 1979
- Passage of phenotypes of chemically transformed cells via transfection of DNA and chromatin.Proceedings of the National Academy of Sciences, 1979
- TRANSFORMATION OF MOUSE C3H-10T1-2 CELLS BY SINGLE AND FRACTIONATED DOSES OF X-RAYS AND FISSION-SPECTRUM NEUTRONS1979
- EFFECTS OF SERUM CONCENTRATION ON EXPRESSION OF CARCINOGEN-INDUCED TRANSFORMATION IN C3H-10T1/2 CL8 CELL LINE1977
- Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol esterNature, 1976
- 2-STAGE CHEMICAL ONCOGENESIS IN CULTURES OF C3H-10T1-2 CELLS1976
- Phenotypic Expression of Transformation: Induction in Cell Culture by a Phorbol EsterScience, 1967
- THE DIFFERENCE IN CONTACT INHIBITION OF CELL REPLICATION BETWEEN NORMAL CELLS AND CELLS TRANSFORMED BY DIFFERENT CARCINOGENSProceedings of the National Academy of Sciences, 1966
- Growth inhibition of polyoma transformed cells by contact with static normal fibroblastsJournal of Cell Science, 1966