Intrinsic labelling of different foods with stable isotope of zinc (67Zn) for use in bioavailability studies

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Abstract
Intrinsically-labelled foods are required to validate extrinsic-labelling techniques used to study the bioavailability of trace elements. Wheat (Triticum aestivum), peas (Pisum sativum), goat's milk, human milk, eggs and chicken meat were selected for intrinsic-labelling studies with67Zn. Peas were grown hydroponically in enriched nutrient solution and wheat was grown in sand and watered with enriched nutrient solution. Some of the wheat plants were also given stem injections of67Zn solution. Eggs and chicken meat were prepared by administering67Zn intravenously to chickens, and human milk was collected after an oral dose of67Zn in a cola drink. All the foods investigated were sufficiently enriched with67Zn for Zn absorption studies except wheat prepared by the sand and water-culture method.