Set of Secondary Emission Standards for Calibration of the Spectral Responsivity in Emission Spectroscopy
- 1 September 1998
- journal article
- research article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 52 (9) , 1179-1189
- https://doi.org/10.1366/0003702981945192
Abstract
Technical spectra are reported for a series of six secondary emission standards based on the commercially available fluorophors: tryptophan, α-NPO, tetraphenylbutadiene, coumarin 153, DCM, and LDS 751. These spectra cover the wavelength range between 300 and 800 nm with significant spectral overlap and conform to the requirements of secondary emission standards. Standard emission spectra are determined by averaging the technical spectra obtained from three independently calibrated fluorescence spectrometers. A method for generating the wavelength-dependent correction file by using these standards is outlined. Comparison to the National Bureau of Standards (NBS)-reported quinine sulfate spectrum illustrates the accuracy of both the emission standards and the method of generating a complete emission correction file. With the use of the standards and method reported here, the accuracy of the emission correction is estimated to be better than 10% for the entire wavelength range and significantly better over the wavelength region of a typical spectrum.Keywords
This publication has 10 references indexed in Scilit:
- Molecular Fluorescence, Phosphorescence, and Chemiluminescence SpectrometryAnalytical Chemistry, 1996
- Correction of Fluorescence SpectraApplied Spectroscopy, 1994
- Standards For Corrected Fluorescence SpectraPublished by SPIE-Intl Soc Optical Eng ,1989
- Removing The Instrument Function From Fluorescence SpectraPublished by SPIE-Intl Soc Optical Eng ,1989
- Spectral irradiance calibrationsPublished by National Institute of Standards and Technology (NIST) ,1987
- Principles of Fluorescence SpectroscopyPublished by Springer Nature ,1983
- Inorganic ions in glasses and polycrystalline pellets as fluorescence standard reference materialsJournal of Research of the National Bureau of Standards Section A: Physics and Chemistry, 1972
- Absolute spectrofluorometryJournal of Research of the National Bureau of Standards Section A: Physics and Chemistry, 1972
- Band Shapes of the Electronic Spectra of Complex MoleculesThe Journal of Chemical Physics, 1969
- Interference Measurements in the Spectra of Neon and Natural MercuryJournal of the Optical Society of America, 1950