ATP binding to bovine serum albumin
- 30 November 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 313 (3) , 288-290
- https://doi.org/10.1016/0014-5793(92)81211-4
Abstract
Specific binding of ATP to bovine serum albumin (BSA) is demonstrated employing ATP derivatives spin-labeled at either N6 or C8 of adenine ring or at the ribose moiety. Based on a 1:1 stoichiometry binding constants are in the 50-100 microM range. Binding is largely competitive with ATP or stearic acid. A small fraction of the labeled nucleotides could not be liberated by these ligands. Binding of AMP is in the millimolar range, only.Keywords
This publication has 22 references indexed in Scilit:
- Synthesis of spin‐labeled 2‐azido‐ATP: Evidence for distinct nucleotide‐binding sites in calcium pump protein from sarcoplasmic reticulumFEBS Letters, 1989
- DiethylstilbestrolEuropean Journal of Biochemistry, 1988
- Synthesis of a photoaffinity-spin-labeled derivative of ATP and its first application to F1-ATPaseFEBS Letters, 1988
- Diethylstilbestrol is a potent inhibitor of the H+-PPase but not of the H+-ATPase of Rhodospirillum rubrum chromatophoresBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1987
- SHORT COMMUNICATIONHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1981
- Analysis of phosphoryl transfer mechanism and catalytic centre geometries of transport ATPase by means of spin-labelled ATPBiochimica et Biophysica Acta (BBA) - Biomembranes, 1980
- Numerical analysis of binding studies: A direct procedure avoiding the pitfalls of a scatchard analysis of equilibrium data for unknown binding modelsInternational Journal of Bio-Medical Computing, 1979
- 8‐spin‐label nicotinamide adenine dinucleotide synthesis and properties of a new spin‐labelled coenzymeFEBS Letters, 1977
- Notiz über Synthese und biochemische Eigenschaften eines Spin‐Label‐Nicotinamid‐adenin‐dinucleotidsEuropean Journal of Organic Chemistry, 1974
- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949