Specificity of antisera obtained to Substance P and its C‐terminal hexapeptide

Publisher Website
Google Scholar
Abstract
Synthetic fragments and analogs were used to characterize specificity of antisera to SP [substance P] and SP6-11. [Tyr8]SP and [Lys6]SP6-11 were both used as radioiodinated ligands. The latter was conjugated with Bolton-Hunter reagents before labeling. In both systems, the C-terminal pentapeptide SP7-11 was the shortest fragment showing antigenic identity with SP molecule. Substitution of different amino acid residues in SP6-11 by His or Gly showed that all but Glu6 take part in the structure of the antigenic determinant. [The identification of spin biological samples using a radioimmunoassay was described.].