EOSINOPHILIA .5. DELAYED-HYPERSENSITIVITY, BLOOD AND BONE-MARROW EOSINOPHILIA, INDUCED IN NORMAL GUINEA-PIGS BY ADOPTIVE TRANSFER OF LYMPHOCYTES FROM SYNGENEIC DONORS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 29  (1) , 75-83
Abstract
Dinitrochlorobenzene (DNCB) induced delayed hypersensitivity [DH] but no eosinophilia in guinea-pigs from 2 colonies. Citraconic anhydride (CA) induced DH and eosinophilia of the blood and bone marrow, and sites of skin tests were also infiltrated by eosinophils. In adoptive transfer of lymphocytes separated from peritoneal exudate cells of strain XIII-sensitized donors, lymphocytes from DNCB-sensitized guinea-pigs transferred antigen-specific DH; lymphocytes from CA-sensitized guinea-pigs transferred DH and induced eosinophilia of the blood and bone marrow of the recipients. Treatment of the lymphocytes before transfer with antilymphocyte (thymocyte) globulin or puromycin suppressed the manifestations in the recipients; normal globulin did not. Active sensitization with DNCB induced formation of small amounts, and with CA larger amounts of anaphylactic antibody. Sera from the actively sensitized animals elicited no significant eosinophilia of blood or bone marrow in 1 group of recipients. Passive anaphylaxis elicited a transient eosinophilia of the blood, but not of the bone marrow. T[thymus-derived]-helper cells probably interact with B[bone marrow-derived]-lymphocyte precursors, particularly Ig[immunoglobulin]E B cells, to stimulate eosinopoeisis. This results in a reserve of mature eosinophils that may be released in any subsequent anaphylactic event.