The Symmetrical Arrangement of Carp α- and β-Globin Genes

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Abstract
The arrangement of carp α-and β-globin genes were examined at the nucleotide sequence level. Then, the nucleotide sequence of 1, 249 base pairs (bp) for the 5' untranslated region of the carp β-globin gene was determined. This region showed a high homology with the previously reported carp a-globin gene No. 7. The overall nucleotide sequence homology of these two clones was 96.7%. DNA sequencing analysis indicated that the 1-globin gene closely linked the α-globin gene. Additionally, the close linkage of carp α-and β-globin genes was proven by Southern blot hybridization and PCR amplification. The direction of the transcription of the α-and β-globin genes was the opposite. The length between the a-and β-globin gene translation initiation site was 792 bp and the nucleotides of this region was high in of A and T contents. Carp α-and β-globin genes show unique symmetrical arrangement.