Ox Erythrocyte Cytotoxicity by Phorbol Myristate Acetate-Activated Human Neutrophils

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Abstract
Human neutrophils activated by phorbol myristatc acelatc were cytoioxic lo ox erythrocyles. as determined by the 51Cr release method. Maximal cytolysis was obtained with a phorbol myristute acetate concentration of 5 ng/ml and with an etTcctor to target cell ratio of 1:4. An inlact neutrophil metabolic burst and production of oxygen-dcrived-frec radicals were essential lor the cytotnxic event, since neulrophils from patients with chronic granulo-malous disease failed to exhibit any ox erythrocyte lysis. The target cell destruction was completely prevented by catalase, was unaffected by supcroxidc dismutase. and was reduced approximately to one-third by azide and cyanide. These data suggest that, under our experimental conditions, the ox erythrocyte killing by phorbol myristatc acetate-activated neutrophils mainly depends on myelopcroxidasc and hydrogen peroxide.