Interference with myosin subfragment‐1 binding by site‐directed mutagenesis of actin
- 1 August 1991
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 200 (1) , 35-41
- https://doi.org/10.1111/j.1432-1033.1991.tb21045.x
Abstract
Three N-terminal double mutants of beta-actin expressed in the yeast Saccharomyces cerevisiae have been characterized with respect to DNase-I interaction, N-terminal post-translational modification, polymerizability and myosin subfragment-1 binding. The results strongly support earlier suggestions that the acidic residues at the N-terminus of actin are part of the myosin-binding site, while they seem to be of no importance for the other aspects of actin biochemistry tested. The suitability of this expression system for production of recombinant actin in general is discussed.Keywords
This publication has 44 references indexed in Scilit:
- Production, isolation and characterization of human profilin fromSaccharomyces cerevisiaeJournal of Muscle Research and Cell Motility, 1991
- Atomic model of the actin filamentNature, 1990
- The effect of divalent cations on the interaction between calf spleen profilin and different actinsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1988
- Improved method for mapping the binding site of an actin-binding protein in the actin sequence. Use of a site-directed antibody against the N-terminal region of actin as a probe of its N-terminusBiochemistry, 1986
- Protein cognitive sites on the surface of actin. A proton NMR studyJournal of Inorganic Biochemistry, 1986
- Site-directed mutagenesis of the regulatory light-chain Ca2+/Mg2+ binding site and its role in hybrid myosinsNature, 1986
- Actin-fragmin interactions as revealed by chemical cross-linkingBiochemistry, 1986
- Chordate muscle actins differ distinctly from invertebrate muscle actinsJournal of Molecular Biology, 1984
- Actin-actin and actin-deoxyribonuclease I contact sites in the actin sequenceBiochemistry, 1984
- At least six different actins are expressed in a higher mammal: An analysis based on the amino acid sequence of the amino-terminal tryptic peptideJournal of Molecular Biology, 1978