Enzyme formation during solid-substrate fermentation in rotating vessels

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Abstract
Aspergillus awamori NRRL 4869 was cultured on the solid substrate, wheat bran, in a modified Rollacell apparatus to produce α‐galactosidase and invertase. The swivel cap on the elongated bottle permits the introduction of air while the bottle rotates. Parameters of air flow rate (0.05–0.2 liter/kg/min), rpm (0.15–15 rpm), and weight of solids (150 and 300 g) were varied. At low air flow rates (0.05 liter/kg solid/min), α‐galactosidase production was minimal independent of the rotation rate. At 0.15 rpm and 0.2 liter/kg solids/min air flow rate, invertase production ceased after five days; whereas α‐galactosidase production continued. The modified Rollacell can be a useful apparatus for studying solid‐substrate cultures.