ACTIVATION OF POLYMORPHONUCLEAR LEUKOCYTES BY SALMONELLA

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Abstract
The interaction of human polymorphonuclear leukocytes (PMN) with Salmonella, as studied by chemiluminescence and phagocytosis, was very different for several clinical isolates. Particularly, bacteria in serogroups C1 and E4 deviated from other Salmonella. The differences were observed in the rate of activation, peak value, duration of chemiluminescence, and extent of association and ingestion, as studied microscopically. Old laboratory S(smooth)-strains such as S. typhimurium 395 MS and S. minnesota S99, which did not associate with the PMN, showed little activation of the PMN; their phagocytosis-sensitive R(rough)-mutants induced rapid activation, high peak values and short duration of chemiluminescence. Certain isolates belonging to the C1/E4 group induced intermediate types of reactions. The kinetics of the activation was related to the physicochemical surface properties of the bacteria. Heating the bacteria at 70.degree. C for 45 min enhanced the activation of PMN by the S-type strains conspicuously, but in different ways; that of R-mutants was delayed. Different clinical isolates of Salmonella showed different physico-chemical surfaces, liability to phagocytosis by PMN and different degrees of eliciting inflammatory mediators from PMN in vitro. Evidently, the C1/E4 group of Salmonella has pathogenicity mechanisms different from most salmonellae.
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