A spectrophotometric study of the ionizations in two ferrihaemoproteins from soya-bean nodules

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Abstract
The ionization of 2 ferrihaemoproteins isolated from soya-bean nodules was followed spectrophotometrically from pH 4 to 9.6, at constant I (0.1). Of the several overlapping haem-linked ionizations so demonstrated, that occurring between pH 6 and 10 is shown to be sufficiently clearly delineated to permit the evaluation of pK under restricted conditions. These conditions are that freshly prepared material be rapidly examined at the gamma peak and that the titration results conform to the criterion that the slope of the linear plot of log ([alkaline pigment]/ [acid pigment)] against pH equals 1.0.