Separation of long-range human TERT gene haplotypes by transformation-associated recombination cloning in yeast
- 24 April 2003
- journal article
- Published by Springer Nature in Oncogene
- Vol. 22 (16) , 2452-2456
- https://doi.org/10.1038/sj.onc.1206316
Abstract
The hTERT gene encoding a catalytic subunit of human telomerase contains four blocks of variable number of tandem repeats (VNTRs)--two in intron 2 and two in intron 6. The segregation of hTERT VNTRs was analysed in families, revealing that all of them were transmitted through meiosis following a Mendelian inheritance. The work reports a further characterization of the minisatellites in hTERT. We employed transformation-associated recombination (TAR) cloning to isolate parental hTERT alleles and determined the specific combination of minisatellites at each of the polymorphic sites. A long-range haplotyping of hTERT determined by TAR cloning was verified by classical Mendelian analysis. Since such a strategy can be applied for any chromosomal locus, we conclude that recombinational gene capture could greatly facilitate haplotypes analysis.Keywords
This publication has 18 references indexed in Scilit:
- A Novel Strategy for Analysis of Gene Homologues and Segmental Genome DuplicationsJournal of Molecular Evolution, 2003
- The human telomerase gene: complete genomic sequence and analysis of tandem repeat polymorphisms in intronic regionsOncogene, 2002
- Rearrangements of minisatellites in the human telomerase reverse transcriptase gene are not correlated with its expression in colon carcinomasOncogene, 2001
- Telomerase activation in colorectal carcinogenesisThe Journal of Pathology, 1999
- The INS 5′ Variable Number of Tandem Repeats Is Associated with IGF2 Expression in HumansJournal of Biological Chemistry, 1998
- A survey of telomerase activity in human cancerPublished by Elsevier ,1997
- Specific Association of Human Telomerase Activity with Immortal Cells and CancerScience, 1994
- Repeat unit sequence variation in minisatellites: A novel source of DNA polymorphism for studying variation and mutation by single molecule analysisPublished by Elsevier ,1990
- Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplificationNature, 1989
- Amplification and analysis of DNA sequences in single human sperm and diploid cellsNature, 1988