Metabolic Processes in Cytoplasmic Particles of the Avocado Fruit. III. The Operation of the Tricarboxylic Acid Cycle.

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Abstract
This paper is concerned with the operation of the tricarboxylic acid cycle in particles obtained by differential centrifugation from avocado fruits. With the aid of manometric and paper chromatographic techniques the oxidation and appearance of the expected products was shown when each of the acids was incubated with the enzyme preparation. The effect of malonate on the inhibition of oxidation and appearance of succinate as product was shown for several acids. The effect of arsenite on the oxidation of the acids and product formation was found to agree with its action on animal mitochondria-inhibiting pyruvate and [alpha]-ketoglutarate oxidation. Co-factor requirements were studied for the oxidation of pyruvate, citrate, [alpha]-ketoglutarate, succinate and malate. Magnesium ions were required in all cases, while the requirement for the other cofactors varied with the substrate used. A sparker quantity of malate was an absolute necessity before any pyruvate oxidation proceeded.

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