Reaction Components Influencing CAMP Factor Induced Lysis

Abstract
The reaction components and conditions affecting CAMP (Christie, Atkins, Munch-Petersen) factor (Streptococcus agalactiae) induced lysis of sheep erythrocyte target cells were investigated. Both the amount of sphingomyelinase used and the time of preincubation with sphingomyelinase directly affected the rate of hemolysis by CAMP factor. The CAMP factor induced lysis was temperature dependent between 15.degree. and 30.degree. C and was proportional to the amount of CAMP factor added.