STUDIES ON LATEX AGGLUTINATION TEST FOR LEPTOSPIROSIS

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Abstract
Experiments were carried out on the applicability of the latex fixation principle for the serological diagnosis of leptospirosis. Polystyrene latex particles suspended in a buffer solution were coated with soluble antigens of Leptospira canicola, L. icterohaemorrhagiae, and L. pomona, respectively, and used as agglutinating antigens for testing sera of various origin. The optimal conditions for the preparation of the test antigens, as well as for the procedure employed in performing the leptospiral latex agglutination test, were determined.The results of the leptospiral latex agglutination test were found to be quite comparable in specificity and sensitivity with those of other serological tests commonly used in laboratory practice. The advantages of the leptospiral latex agglutination test over some other serological tests are found in the simplicity and rapidity of its performance and in the use of a non-infective, stable antigen, which eliminates the necessity of maintaining live leptospiral cultures in diagnostic laboratories.

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