Studies on the Regulation of X-Prolyl Dipeptidyl Aminopeptidase Activity

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Abstract
The specific activity of X-prolyl-dipeptidyl aminopeptidase in Saccharomyces cerevisiae grown on glucose-containing medium remains constant during exponential growth and increases less than 2-fold when cells reach the stationary phase. In cells harvested from exponential growth on glucose-containing medium, the specific activity of the enzyme is found to be 20-30% lower than the specific activity observed in media without glucose, containing acetate or ethanol as the C source. X-Prolyl-dipeptidyl aminopeptidase is not inactivated after the addition of glucose to stationary phase cells. Growth of the yeast on poor N sources or under N-starvation results in a 3- to 4-fold increase in the level of the enzyme.