A TECHNIQUE FOR THE DEMONSTRATION OF ACETYLCHOLINESTERASE ACTIVITY IN THE INNER EAR AFTER DECALCIFICATION WITH EDTA
- 1 December 1964
- journal article
- letter
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 12 (12) , 931-933
- https://doi.org/10.1177/12.12.931
Abstract
The petrous bones of adult cats and guinea pigs were decalcified with buffered cold Na-ethylenediaminetetraacetate and then cut in a cryostat at -18[degree]C. The frozen sections were used for the histochemical demonstration of acetycholinesterase or butyrl-cholinesterase activity with the thiocholine method as modified by Gomori. Enzyme activity was localized in the ganglion cells, nerves and nerve endings after 40 days of decalcification.Keywords
This publication has 2 references indexed in Scilit:
- FURTHER OBSERVATIONS ON OXIDATIVE ENZYME ACTIVITIES IN DECALCIFIED BONE AND TEETHJournal of Histochemistry & Cytochemistry, 1964
- DECALCIFICATION WITH VERSENE FOR HISTOCHEMICAL STUDY OF OXIDATIVE ENZYME SYSTEMSJournal of Histochemistry & Cytochemistry, 1962