Estimation of Intracellular Free Triiodothyronine in Man*
- 1 April 1980
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 50 (4) , 667-669
- https://doi.org/10.1210/jcem-50-4-667
Abstract
(T)3 RIA and measurements of dialyzable fraction Ta (DFT3) have been applied to human erythrocyte (RBC) cytoplasm in order to quantitate the intracellular free T3(FT3) concentration in man. Mean intracellular (T3, DFT3) and FT3 in RBCs from four normal subjects were, respectively, 12 ngdl, 1.25%, and 0.15 ng/dl. These values were 10%, 450%, and 44% (those of T3, DFT3, and FT3 in plasma obtained from the same subjects. In RBCs from four hyperthyroid subjects, the meancytosol (DFT)3 was slightly increased compared to euthyroid (RBCs. Because intracellular T3 concentration was increased 8-fold, however, the cytosol FT3 was frankly elevated relative to euthyroid state cells. Thus, intracellular T3 and FT3 measurements have been shown to be feasible in the human RBC and (confirm that increases in extracellular free T3 are in fact accom- panied by elevations of intracellular free hormone.Keywords
This publication has 3 references indexed in Scilit:
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- Determination of Triiodothyronine in Red Blood Cells by RadioimmunoassayEndocrinologia Japonica, 1976
- Proteins Associated with the Thyroid HormonesPhysiological Reviews, 1960