CELLS MEDIATING GRAFT REJECTION IN THE MOUSE II. THE LY PHENOTYPES OF CELLS PRODUCING TUMOR ALLOGRAFT REJECTION

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Abstract
The particular T cell subsets involved in the rejection of tumor allografts were examined in mice using 2 different approaches. In the 1st, EL4 lymphoma cells or B16 melanoma cells were given to ATXBM-CBA/H mice which had been reconstituted with either naive or sensitized T cells selectively depleted with either Ly-1 or Ly-2 monoclonal antibodies. In mice receiving nonsensitized T cells, Ly-123 cells and to a lesser extent Ly-1 cells were involved in the rejection of both tumors; Ly-23 cells played no role in this rejection. In mice receiving sensitized cells, rejection was mediated by Ly-1 cells and not by Ly-123 or Ly-23 cells. In a 2nd approach, sensitized cells were mixed with the tumor cells prior to injection into mice (the Winn neutralization assay). In this case, Ly-123 were the prime mediators of graft rejection. Both Ly-1 and Ly-123 cells can apparently act as mediators of graft rejection. Ly-1-sensitized cells probably act as in a delayed-type hypersensitivity (DTH) response where few lymphocytes can lead to the rejection of many cells. Ly-123 cells can act as killer T cells but large numbers and the close apposition with the target cells are required. A requirement for Ly-123 precursor cells in nonsensitized mice, probably acting as precursors for both types of effector cell, is demonstrated.