The effect of culture conditions and toxicity on the ames salmonella/microsome agar incorporation mutagenicity assay

Abstract
Various methods were used to reconstruct an environment similar to a chemically bactericidal or bacteriostatic effect in the Ames mutagenicity assay in order to determine whether a false‐positive result could occur. Neither population reduction, sonication, nor increased histidine produced an increase of spurious revertant colonies.