Properties of the penicillin-binding proteins of four species of the genus Bacteroides
Open Access
- 1 May 1986
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 29 (5) , 825-832
- https://doi.org/10.1128/aac.29.5.825
Abstract
The penicillin-binding proteins (PBPs) of four species of the genus Bacteroides were examined in cell envelope preparations from exponentially growing cultures and intact cells. Upon examination by sodium dodecyl sulfate-polyacrylamide electrophoresis, three major high-molecular-weight PBPs (molecular weight, 58,000 to 82,000) were resolved, and low-molecular-weight PBPs were seen in all strains except Bacteroides fragilis. The sporadic appearance of PBP 4 in B. fragilis (molecular weight, approximately 45,000) was shown not to be influenced by the concentration of free iron available in the medium or by the stage of growth at which the batch culture was harvested. No PBP that was inhibited by an aerobic environment was demonstrated. The affinity of 35 beta-lactam antibiotics for the PBPs from envelope preparations was examined and correlated with the morphological response. Most compounds bound initially to PBP 2 and then PBP 1, correlating with a primary response of filamentation and then spheroplasting and lysis. Compounds such as clavulanic acid bound to PBP 3 at concentrations causing round cells. Based on the data from this study, it is proposed that the three high-molecular-weight PBPs of Bacteroides fragilis, Bacteroides vulgatus, Bacteroides thetaiotaomicron, and Bacteroides ovatus correlate to the three high-molecular-weight PBPs of Escherichia coli and that the PBPs of Bacteroides species perform the same enzymic role in cell wall biosynthesis as their counterparts in E. coli. Therefore, the components of PBP 1 are involved in cell elongation, PBP 2 is involved in septum formation, and PBP 3 is involved in maintenance of cell shape (i.e., PBP 2 in Bacteroides spp. = PBP 3 in E. coli, and PBP 3 in Bacteroides spp. = BPB 2 in E. coli).This publication has 30 references indexed in Scilit:
- The morphological response of Pseudomonas aeruginosa to azthreonam, cefoperazone, ceftazidime and N-formimidoyl thienamycinJournal of Medical Microbiology, 1984
- Mode of action of ceftazidime: affinity for the penicillin-binding proteins of Escherichia coli K12, Pseudomonas aeruginosa and Staphylococcus aureusJournal of Antimicrobial Chemotherapy, 1983
- PENICILLIN-BINDING PROTEINS AND THE MECHANISM OF ACTION OF BETA-LACTAM ANTIBIOTICSAnnual Review of Biochemistry, 1983
- Penicillin-binding proteins in Bacteroides fragilis and their affinities for several new cephalosporinsJournal of Antimicrobial Chemotherapy, 1983
- Benzylpenicillin-induced Filament Formation of Clostridium perfringensMicrobiology, 1982
- Antibacterial activity of new β-lactam antibiotics on cefoxitin-resistant strains of Baeteroides fragilisJournal of Antimicrobial Chemotherapy, 1980
- Comparative Studies of Penicillin-Binding Proteins in Pseudomonas aeruginosa and Escherichia coliEuropean Journal of Biochemistry, 1979
- The Separation and Properties of Two Penicillin-Binding Proteins from Salmonella typhimuriumEuropean Journal of Biochemistry, 1977
- Mutants of Escherichia coli which lack a component of penicillin‐binding protein 1 are viableFEBS Letters, 1977
- Properties of the Penicillin‐Binding Proteins of Escherichia coli K12European Journal of Biochemistry, 1977