Complementation of the Magnaporthe grisea ΔcpkA Mutation by the Blumeria graminis PKA-c Gene: Functional Genetic Analysis of an Obligate Plant Pathogen
Open Access
- 1 December 2001
- journal article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 14 (12) , 1368-1375
- https://doi.org/10.1094/mpmi.2001.14.12.1368
Abstract
Obligate plant-pathogenic fungi have proved extremely difficult to characterize with molecular genetics because they cannot be cultured away from host plants and only can be manipulated experimentally in limited circumstances. Previously, in order to characterize signal transduction processes during infection-related development of the powdery mildew fungus Blumeria graminis (syn. Erysiphe graminis) f. sp. hordei, we described a gene similar to the catalytic subunit of cyclic AMP-dependent protein kinase A (here renamed Bka1). Functional characterization of this gene has been achieved by expression in a deltacpkA mutant of the nonobligate pathogen Magnaporthe grisea. This nonpathogenic M. grisea deltacpkA mutant displays delayed and incomplete appressorium development, suggesting a role for PKA-c in the signal transduction processes that control the maturation of infection cells. Transformation of the deltacpkA mutant with the mildew Bka1 open reading frame, controlled by the M. grisea MPG1 promoter, restored pathogenicity and appressorium maturation kinetics. The results provide, to our knowledge, the first functional genetic analysis of pathogenicity in an obligate pathogen and highlight the remarkable conservation of signaling components regulating infection-related development in pathogenic fungi.Keywords
This publication has 27 references indexed in Scilit:
- Gene Identification in the Obligate Fungal Pathogen Blumeria graminis by Expressed Sequence Tag AnalysisFungal Genetics and Biology, 2001
- MAP Kinase and Protein Kinase A–Dependent Mobilization of Triacylglycerol and Glycogen during Appressorium Turgor Generation by Magnaporthe griseaPlant Cell, 2000
- Colletotrichum trifoliiMutants Disrupted in the Catalytic Subunit of cAMP-Dependent Protein Kinase Are NonpathogenicMolecular Plant-Microbe Interactions®, 1999
- Regulation of cAMP-dependent protein kinase during appressorium formation in Magnaporthe griseaFEMS Microbiology Letters, 1999
- Divergent cAMP Signaling Pathways Regulate Growth and Pathogenesis in the Rice Blast Fungus Magnaporthe griseaPlant Cell, 1998
- The adenylate cyclase gene MAC1 of Magnaporthe grisea controls appressorium formation and other aspects of growth and development.Plant Cell, 1997
- Hydrolytic Enzymes of Ungerminated and Germinated Conidia of Erysiphe graminis DC f. sp. hordei MarchalJournal of Phytopathology, 1994
- Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea.Plant Cell, 1993
- cAMP Regulates Infection Structure Formation in the Plant Pathogenic Fungus Magnaporthe grisea.Plant Cell, 1993
- Restoration of Appressorial Penetration Ability by Melanin Precursors in Pyricularia oryzae Treated with Antipenetrants and in Melanin-Deficient MutantsJournal of Pesticide Science, 1987