cDNA cloning, expression and primary structure of Par j I, a major allergen of Parietaria judaica pollen
Open Access
- 21 March 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 341 (2-3) , 182-186
- https://doi.org/10.1016/0014-5793(94)80453-2
Abstract
A 659 bp cDNA clone1 coding for an allergen of Pj pollen has been isolated from a lambda gt11 library, and its DNA sequence determined. The cDNA insert showed an open reading frame of 429 bp coding for an allergenic protein of 14,866 Da and a deduced amino acid sequence containing 143 residues. The expressed recombinant protein represented the major allergen Par j I since it reacted with 95% of the sera from Pj‐allergic patients (n = 22) and with two Par j I‐specific monoclonal antibodies. No similarity with other known DNA and protein sequences has been detected.Keywords
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